Journal: Clinical and Vaccine Immunology : CVI

Article Title: Immunoproteomic Identification of In Vivo -Produced Propionibacterium acnes Proteins in a Rabbit Biofilm Infection Model

doi: 10.1128/CVI.00760-14

Figure Lengend Snippet: Schematic flow chart of the implant-associated osteomyelitis model in rabbits. After a 5-day incubation of the P. acnes strain RMA 13884 with dextran beads, rabbits were infected with a P. acnes inoculum in the left tibia. After 4 weeks of infection, blood serum was isolated, and X rays of both tibias were performed. The tibias were macroscopically and histologically examined, and the bacterial loads were quantified and reported as CFU per g of bone.

Article Snippet: SEM studies confirmed biofilm formation in both P. acnes strains (ATCC 11827 and RMA 13884) incubated with soda-lime solid-glass beads for 2 days at 37°C under static conditions. shows a young P. acnes (ATCC 11827 strain) biofilm with embedded cells within an exopolymeric matrix that appears similar to the glycocalyx of the biofilms of staphylococci. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 Scanning electron micrograph (SEM) of the P. acnes strain ATCC 11827 biofilm on solid glass beads (Walter Stern, Inc., Port Washington, NY) incubated for 2 days anaerobically at 37°C under static conditions (magnification, 2,000× [left panel] and 20,000× [right panel]), using a Zeiss Supra 55VP field emission scanning electron microscope with a beam accelerating voltage of 1 kV and a working distance of 3 mm.

Techniques: Incubation, Infection, Isolation